ISOLASI DAN IDENTIFIKASI BAKTERI GRAM NEGATIF PADA AMBING KAMBING PERANAKAN ETAWA (PE) (Isolation and Identification of Gram Negative Bacteria on the Udder of Etawa Crossbred (PE) Goat)

Septian Tri Mulyana Ginting, Teuku Zahrial Helmi, Darmawi Darmawi, Hennivanda Hennivanda, Erina Erina, Razali Daud

Abstract


ABSTRAK

Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Gram negatif pada ambing kambing peranakan etawa (PE). Sampel yang digunakan pada penelitian ini terdiri dari swab ambing dari 5 ekor kambing PE. Sampel dibiakkan pada media nutrient broth lalu diinkubasikan pada suhu 37°C selama 24 jam. Isolasi dan identifikasi dilakukan dengan metode Carter yang meliputi pengamatan morfologi koloni, pewarnaan Gram, penanaman pada media diferensial dan selektif serta uji biokimia. Hasil isolasi dan identifikasi pada 5 sampel swab ambing kambing PE didapatkan dua koloni bakteri Gram negatif yaitu Escherichia coli dan bakteri X yang belum teridentifikasi melalui uji-uji yang dilakukan. Berdasarkan hasil tersebut dapat disimpulkan bahwa bakteri Gram negatif pada ambing kambing PE adalah Escherichia coli dan bakteri X yang belum teridentifikasi.

Kata kunci: kambing PE, ambing, bakteri Gram negatif.

 

ABSTRACT

This study aimed to isolate and identify Gram-negative bacteria on udder of etawa crossbred (PE) goat. Swab samples were obtained from 5 PE goats udder. Swab samples were cultured in nutrient broth media and incubated at 37°C for 24 hours. Isolation and identification were performed by Carter method, followed by observation of colony morphology, Gram staining, cultured on differential and selective media, and biochemical tests were performed. The result showed that there were two colonies of Gram negative bacteria Escherichia coli and the other colony has not been identified yet with this tests. Therefore it can be concluded that the Gram-negative bacteria found on udder of PE goat were Escherichia coli and bacteria X that has not been identified by using this method.

Keywords:etawa crossbred goat, udder, Gram-negative bacteria.

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DOI: http://dx.doi.org/10.21157/jim%20vet..v2i3.8206

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