Jumlah Koloni Bakteri Selulolitik pada Sekum Ayam Kampung (Gallus domesticus)

raudhatul jannah jannah, Safika Safika, Muhammad Jalaluddin

Abstract


Penelitian ini bertujuan menghitung jumlah koloni bakteri selulolitik pada sekum ayam kampung. Sampel yang digunakan yaitu isi sekum ayam kampung yang diambil di Tempat Pemotongan Unggas (TPU) Lambaro Aceh Besar sebanyak 5 ekor ayam kampung. Sampel dilakukan pengenceran 10-2 sampai 10-5, kemudian diinokulasi ke dalam media BHM-CMC agar dengan metode tuang (pour plate). Selanjutnya, diinkubasi dengan suhu 37˚C selama 96 jam. Koloni bakteri selulolitik yang tumbuh kemudian dilakukan pengamatan morfologi koloni (warna, posisi, pinggiran, dan diameter koloni), diameter zona bening, penghitungan jumlah total bakteri, dan pewarnaan Gram. Hasil penelitian menunjukkan bahwa koloni bakteri berwarna putih, berada di dalam dan di permukaan agar, memiliki pinggiran rata, diameter koloni 2-3 mm dan diameter zona bening 18 mm dan 29 mm. Bakteri berbentuk basil bereaksi negatif terhadap pewarnaan Gram, sedangkan bakteri berbentuk kokus bereaksi positif dan negatif terhadap pewarnaan Gram. Jumlah total bakteri pada isi sekum ayam kampung yaitu 5.3 x 105 cfu/g.

Kata kunci: Ayam kampung, bakteri selulolitik, sekum, Carboxy Methyl Cellulase.

                The aim of this study was to count the total count of cellulolytic bacteria colony in caecum of native chicken. The sample used was caecum contents of native chicken which taken from the poultry slaughterplace at Lambaro Aceh Besar. Sample were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. Then, incubated with temperature 37oC about 96 hours. A colony of cellulolytic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of the colony), diameter of the bacterial zone, counting a total of bacteria which was grown, and Gram stain. The results showed that there is white bacterial colony, located inside and surface agar, has flat periphery, diameters of colony 2-3 mm and diameters of bacterial zone 18 and 29 mm. Bacill-shaped bacteria react negative to Gram staining, whereas coccus-shaped bacteria react positive and negative to Gram staining. Number of total bacteria in the native chicken’s caecum was 5.3 x 105 cfu/g

 

Keywords: Native chicken, cellulolytic bacteria, caecum, Carboxy Methyl Cellulose.


Keywords


Kata kunci: Ayam kampung, bakteri selulolitik, sekum, Carboxy Methyl Cellulase.

Full Text:

PDF

References


Alam, M.Z., Manchur M.A, and M.N. Anwar. 2004. Isolation, purification, characterization of cellulolytic enzymes produced by Streptomyces omyaensin. J. Biol. Sci.10(2):1647-1653.

Anand, Vennison, Sankar, Prabhu, Vasan, Raghuraman, Geoffrey, and Vendan. 2009. Isolation and characterization of bacteria from the gut of Bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion. Journal of Insect Science. 107(10):1-20.

Anggorodi, R. 1985. Kemajuan Mutakhir dalam Ilmu Makanan Ternak Unggas. Cetakan ke-1.Universitas Indonesia Press, Jakarta.

Anuar, W., A. Dahliaty, dan C. Jose. 2014. Isolasi bakteri selulolitik dari perairan Dumai. Jurnal of Mipa. 1(2):3-6.

Apajalahti, J., A. Kettunen, and H. Graham. 2004. Characteristic of the gastrointestinal microbial communities with special reference to the chicken. J. Poultry Sci. 60(1):223-232.

Astuti, N. 2012. Kinerja ayam kampung dengan ransum berbasis konsentrat ayam broiler. Jurnal Agrisains. 4(5):1-2.

Denbow, D.M. 2000. Gastrointestinal Anatomy and Physiology. Academic Press, London.

Febriyossa, A., Nurmiati, dan Periadnadi. 2013. Potensi dan karakterisasi bakteri alami pencernaan ayam broiler pedaging (Gallus gallus domesticus L.) sebagai kandidat probiotik pakan ayam broiler. Jurnal Biologi Universitas Andalas. 2(3):201-106.

Hadioetomo R.S. 1993. Mikrobiologi Dasar Dalam Praktek Teknik dan Prosedur Dasar Laboratorium. Penerbit Gramedia, Jakarta.

Hartanti. 2010. Isolasi dan Seleksi Bakteri Selulolitik Termofilik dari Kawah Air Panas Gunung Pancar Bogor. Skripsi. FMIPA, Institut Pertanian Bogor, Bogor.

Ibrahim, A.S.S, and A. Dewany. 2007. Isolation and identification of new cellulases producing thermophilic bacteria from an egyptian hot spring and some properties of the crude enzyme. Australian Journal of Basic and Applied Sciences. 4(1):473-478.

Pelczar, M.J. dan E.C.S. Chan. 2009. Dasar Dasar Mikrobiologi. Penterjemah: R.S. Hadioetomo, T. Imas dan S.S Tjitrosomo. Edisi 2. UI Press, Jakarta.

Pratiwi, S.T. 2008. Mikrobiologi Farmasi. Penerbit Erlangga, Yogyakarta.

Rahayu, A.G., Y. Haryani, dan F. Puspita. 2014. Uji aktivitas selulolitik dari tiga isolat bakteri Bacillus sp galur lokal Riau. Jurnal of Mipa.1(2):3-4.

Rao, S.N.S. 1994. Mikroba Tanah dan Pertumbuhan Tanaman. Edisi 2. Universitas Indonesia Press, Jakarta.

Saratale, G.D. 2012. Production and characterization of multiple cellulolytic enzymes by isolated Streptomyces sp. Biomass and Bioenergy. 47(6):302-315.

Spring, P. 1997. Understanding the Development of the Avian Gastrointestinal Microflora. In : an essential key for developing competitive exclusion products. Pp. 149-160. University of Kentucky, Lexington.

Varastegani, A. and Dahlan. 2014. Influence of dietary fiber levels on feed utilization and growth performance in poultry. J. Anim. Pro. Adv. 4(6):422-429.

Waluyo, L. 2007. Mikrobiologi Umum. Universitas Muhamadiyah Malang, Malang.

Yosmar, R., N. Suharti, dan R. Rasyid. 2013. Isolasi dan uji kualitatif hidrolisat jamur penghasil enzim selulase dari tanah tumpukan ampas tebu. Jurnal Farmasi Andalas. 1(1):2-4.




DOI: http://dx.doi.org/10.21157/jim%20vet..v1i3.4023

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 JURNAL ILMIAH MAHASISWA VETERINER

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.